Heterogeneous Nucleation of Protein Crystals on Fluorinated Layered Silicate

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Heterogeneous Nucleation of Protein Crystals on Fluorinated Layered Silicate

Here, we describe an improved system for protein crystallization based on heterogeneous nucleation using fluorinated layered silicate. In addition, we also investigated the mechanism of nucleation on the silicate surface. Crystallization of lysozyme using silicates with different chemical compositions indicated that fluorosilicates promoted nucleation whereas the silicates without fluorine did ...

متن کامل

Heterogeneous crystal nucleation on bubbles in silicate melt

Experiments reported herein document heterogeneous crystal nucleation on bubbles in supercooled lithium disilicate melt. Crystalline lithium disilicate (Li2Si2O5) nucleated and grew on small bubbles (;1 mm) with a one-to-one correspondence between the number of bubbles and crystals (ranging from ,102 to ;105 bubbles/mm3). Crystals grew on large bubbles (.100 mm) only in samples fused in N2, sug...

متن کامل

Nucleation of protein crystals.

This paper introduces nucleation theory applied to crystallizing protein solutions. It is shown that the classical approach explains the available nucleation data under most conditions used for growing protein crystals for structural studies and for industrial crystallization. However, it fails to explain most experimental data on the structure of the critical clusters. It is also shown that fo...

متن کامل

Protein crystals and charged surfaces: interactions and heterogeneous nucleation.

As proteins typically have charges of around 10, they will interact strongly with charged surfaces. We calculate the electrostatic contribution to the interaction of crystals of protein with charged surfaces. The surfaces repel like-charged crystals and attract oppositely charged crystals, with free energies that can be easily several kT per protein molecule brought into contact with the surfac...

متن کامل

Experiment and theory for heterogeneous nucleation of protein crystals in a porous medium.

The determination of high-resolution structures of proteins requires crystals of suitable quality. Because of the new impetus given to structural biology by structural genomics/proteomics, the problem of crystallizing proteins is becoming increasingly acute. There is therefore an urgent requirement for the development of new efficient methods to aid crystal growth. Nucleation is the crucial ste...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: PLoS ONE

سال: 2011

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0022582